Coding

Part:BBa_K2415003:Design

Designed by: Yunping Lin   Group: iGEM17_GZHS-United   (2017-10-21)


MTX1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1970
    Illegal BamHI site found at 2009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1269


Design Notes

The sequence was codon optimized and is suitable for protein expression in E.coli.

Source

It was synthesized by IDT.

References